Evaluation of the magnetic field requirements for nanomagnetic gene transfection
نویسندگان
چکیده
UNLABELLED The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. CONCLUSION In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™.
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